Abstract
The genus Marrubium, belonging to the family Lamiaceae, is highly praised in herbal medicine of different countries for having renowned healing properties. Herein, the anti-inflammatory and anti-angiogenesis potential of Marrubium persicum methanol extract was evaluated in a mouse air pouch model of inflammation. Aerial parts of M. persicum were solvent extracted using the Soxhlet apparatus. Subsequently, air injections were performed (for 3 days) into the mice's backs to bring about an air pouch, while carrageenan was used to induce inflammation. The mice were divided into four groups, including; negative control (normal saline into the pouch), control (carrageenan), treatment and positive control (dexamethasone). The inflammatory markers were analyzed 48h after injecting carrageenan, and a haemoglobin assay kit assessed the quantification of angiogenesis in granulation tissue. M. persicum methanol extract at doses of 3.5, 5, 7.5 and 10 mg/kg represented significant decreases in inflammatory parameters. Compared to the control group, the optimum dose (3.5 mg/kg) lessened the myeloperoxidase (MPO) and angiogenesis activity, as well as haemoglobin levels. In sum, the methanol extract of M. persicum exhibited anti-inflammatory effects against carrageenan-induced inflammation, which could be related to its antioxidant and inhibitory effects on neutrophils' infiltration.