Direct evidence for poison use on microlithic arrowheads in Southern Africa at 60,000 years ago

Poisoned weapons are a hallmark of advanced hunter-gatherer technology. Through targeted microchemical and biomolecular analyses, we identified traces of toxic plant alkaloids on backed microliths from Umhlatuzana Rock Shelter in KwaZulu-Natal, South Africa, excavated from a level dated to 60,000 years ago. The alkaloids buphandrine and epibuphanisine only originate from Amaryllidaceae indigenous to southern Africa. The most likely source is Boophone disticha (L.f.) Herb. bulb exudate, also associated with historically documented arrow poisons. To our knowledge, we present the first direct evidence for the application of this plant-based poison on the tips of Pleistocene hunting weapons. The discovery highlights the complexity of subsistence strategies and cognition in southern Africa since the mid-Pleistocene.