Abstract
Astragalus scaberrimus Bunge 1833 is a widespread perennial herb in northern China. The plant has white flowers and white hairs on the leaves and stems. To determine the chloroplast genome, total DNA was extracted from a sample and sequenced on the Illumina HiSeq4000 platform. After sequencing, the reads of chloroplast DNA were assembled and annotated via NOVOPlasty and PGA respectively. The chloroplast genome of this plant has a circular form with a length of 123,492 bp, a 34% GC content and IR loss. After annotation, a total of 113 genes were predicted for this cp genome, comprising 79 encoded proteins, 4 rRNAs and 30 tRNAs. The evolutionary history indicates that A. scaberrimus was grouped within Astragalus and formed a clade with Astragalus laxmannii with a 100% BS support value. The complete chloroplast genome can serve as a reference for future studies on molecular biology, evolution, population genetics, taxonomy and resource protection.