Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography versus Ultracentrifugation
从人卵泡液中分离细胞外囊泡：尺寸排阻色谱法与超速离心法
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作者：Maria Soares, Maria M Pinto, Rui Jorge Nobre, Luís Pereira de Almeida, Maria da Graça Rasteiro, Teresa Almeida-Santos, João Ramalho-Santos, Ana Paula Sousa
| 期刊： | Biomolecules | 影响因子： | 4.800 |
| 时间： | 2023 | 起止号： | 2023 Feb 2;13(2):278. |
| doi： | 10.3390/biom13020278 | 种属： | Human |
| 方法学： | FCM、IF、IHC-P、WB | 靶点： | LAMP2 |
| 研究方向： | 囊泡、细胞生物学 | 细胞类型： | 其它细胞 |
Methods
size-exclusion chromatography (SEC) and ultracentrifugation (UC). EVs isolated using SEC and UC were compared regarding their size and concentration using dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA); protein contamination was assessed with microBCA; specific EV markers were detected with Western blot, and EV morphology was studied with transmission electron microscopy (TEM). Our
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