Conclusions
MiR-141 may promote proliferation and reduce apoptosis of colon cancer cells via targeting Sirt1.
Methods
The samples of stage I, II, III and IV colon cancer were obtained, and the miRNA expression levels was analyzed, with normal colon tissues as controls. The expression of miR-141 and miR-34 was detected via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and the cell proliferation and apoptosis in each group were detected via cell counting kit-8 (CCK8) assay, respectively. Finally, the protein expressions of Sirt1, Caspase-3 and Caspase-8 were determined using Western blotting.
Purpose
To explore the effects of micro ribonucleic acid-141 (miR)-141 on the proliferation and apoptosis of colon cancer cells and its association with the sirtuin 1 (Sirt1) expression.
Results
The expressions of miR-141 and miR-34 (miR-34 is mentioned in previous methods. Furthermore, we found the expression of miR-141 increasing with the progression of colon cancer, which was higher in stage III than in stage I-II and also higher in stage IV than in stage III. miR-34 was also highly expressed in stage IV colon cancer in our study were up-regulated in the progression of colon cancer. Overexpression of miR-141 could promote cell proliferation (p<0.05) and inhibit apoptosis (p<0.05), while inhibition on miR-141 expression could significantly weaken cell proliferation (p<0.05) and promote apoptosis (p<0.05). The results of luciferase reporter assay showed that miR-141 obviously inhibited Sirt1 (p<0.05). SRT2183 reduced cell proliferation (p<0.05) but up-regulated the protein expressions of Sirt1, Caspase-3 and Caspase-8 (p<0.05), while EX 527 had the opposite effects (p<0.05). Conclusions: MiR-141 may promote proliferation and reduce apoptosis of colon cancer cells via targeting Sirt1.