3D Bioprinted Neural Tissues: Emerging Strategies for Regeneration and Disease Modeling

3D生物打印神经组织:再生和疾病建模的新兴策略

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Abstract

Three-dimensional (3D) bioprinting has emerged as a versatile platform in regenerative medicine, capable of replicating the structural and functional intricacies of the central and peripheral nervous systems (CNS and PNS). Beyond structural repair, it enables the construction of engineered tissues that closely recapitulate neural microenvironments. This review provides a comprehensive and critical synthesis of current bioprinting strategies for neural tissue engineering, with particular emphasis on comparing natural, synthetic, and hybrid polymer-based bioinks from mechanistic and translational perspectives. Distinctively, it highlights gradient-based modulation of Schwann cell behavior and axonal pathfinding using mechanically and chemically patterned constructs. Special attention is given to printing modalities such as extrusion, inkjet, and electrohydrodynamic jet printing, examining their respective capacities for controlling spatial organization and microenvironmental cues. Representative applications include brain development models, neurodegenerative disease platforms, and glioblastoma scaffolds with integrated functional properties. Furthermore, this review identifies key translational barriers-including host tissue integration and bioink standardization-and explores emerging directions such as artificial intelligence-guided biofabrication and organ-on-chip integration, to enhance the fidelity and therapeutic potential of neural bioprinted constructs.

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