Abstract
In recent years, the calcifying properties of some cyanobacteria have been used in the production of living building materials (LBMs), such as bio-concrete, as a CO(2)-friendly alternative for cement. This microbially induced calcium carbonate precipitation (MICP) technique can act as a novel platform technology for carbon capture strategies. Consequently, various research articles have been conducted based on a diverse set of workflows, including several modifications, to manufacture LBMs. However, such articles contain only fragmentary descriptions of the materials and methods used. This protocol is meant to act as a detailed, step-by-step operational manual for the production of LBMs using the cyanobacterial model strain Picosynechococcus sp. PCC 7002. The process is divided into several steps, such as the activation of the cyanobacterial-gel solution with CaCl(2) × 2H(2)O and NaHCO(3), casting standardized prisms (160 × 40 × 40 mm), and demolding LBMs. Subsequently, bending tensile and compressive strength tests are performed according to the procedures commonly used in concrete and material testing as proof of concept. Key features • A comprehensive workflow for the manufacturing of cement-free living building materials with cyanobacteria. • A cyanobacteria-gelatin-containing solution is activated, mixed with sand, casted, curated, and strength tested. • Adaptable for other cyanobacterial strains and substitute materials.