Abstract
This study reports the first records of the genus Shannoniana Lane & Cerqueira, 1942 and Trichoprosopon aff. castroi Lane & Cerqueira, 1942 in Peru, both belonging to the Sabethini tribe of mosquitoes. Fieldwork was conducted in the Cerro Adobe conservation center in the Amazonas region, where ovitraps were used to collect mosquito larvae, which were then reared in the laboratory for identification. Morphological and molecular analyses confirmed the presence of these species, with Shannoniana showing differences from previously described species, and Trichoprosopon aff. castroi being recognized as a new record of this species for Peru. Phylogenetic analysis of COI sequences revealed 3 distinct genetic lineages, supporting the monophyly of Sabethini but indicating inconsistencies in the internal relationships within the genus Trichoprosopon. This work expands the known diversity of Sabethini mosquitoes in Peru and underscores the value of molecular tools in resolving taxonomic challenges in mosquito studies.