Abstract
Aesculus pavia L., a member of the genus Aesculus in the family Sapindaceae, holds significant value as both a medicinal and ornamental plant. In this study, we assembled and annotated the complete chloroplast genome of A. pavia and conducted the phylogenetic analysis among the genus Aesculus. The complete chloroplast genome of A. pavia is 156,394 bp in length, with a GC content of 37.90%. It exhibits a typical quadripartite structure, consisting of a large single-copy (LSC) region (85,927 bp), a small single-copy (SSC) region (18,751 bp), and a pair of inverted repeats (IRs) regions (25,858 bp). A total of 133 genes were annotated, including 88 protein-coding genes (PCG), 37 tRNA genes, and 8 rRNA genes. Phylogenetic analyses revealed that A. pavia is clustered with A. turbinata and A. hippocastanum, suggesting a close relationship between A. pavia and the two species. This study provides a new plastome sequence for evolutionary and phylogenetic studies of the genus Aesculus.