Abstract
A Gram-negative, aerobic, rod-shaped, motile bacterium with multi-flagella, strain RS(T), was isolated from bacterial wilt of tobacco in Yuxi city of Yunnan province, China. The strain contains the major fatty acids of C(16:0), summed feature 3 (C(16:1) ω7c and/or C(16:1) ω6c), and summed feature 8 (C(18:1) ω7c and/or C(18:1) ω6c). The polar lipid profile of strain RS(T) consists of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and unidentified aminophospholipid. Strain RS(T) contains ubiquinones Q-7 and Q-8. 16S rRNA gene sequence (1,407 bp) analysis showed that strain RS(T) is closely related to members of the genus Ralstonia and shares the highest sequence identities with R. pseudosolanacearum LMG 9673(T) (99.50%), R. syzygii subsp. indonesiensis LMG 27703(T) (99.50%), R. solanacearum LMG 2299(T) (99.28%), and R. syzygii subsp. celebesensis LMG 27706(T) (99.21%). The 16S rRNA gene sequence identities between strain RS(T) and other members of the genus Ralstonia were below 98.00%. Genome sequencing yielded a genome size of 5.61 Mbp and a G + C content of 67.1 mol%. The genomic comparison showed average nucleotide identity (ANIb) values between strain RS(T) and R. pseudosolanacearum LMG 9673(T), R. solanacearum LMG 2299(T), and R. syzygii subsp. indonesiensis UQRS 627(T) of 95.23, 89.43, and 91.41%, respectively, and the corresponding digital DNA-DNA hybridization (dDDH) values (yielded by formula 2) were 66.20, 44.80, and 47.50%, respectively. In addition, strains belonging to R. solanacearum phylotype I shared both ANIb and dDDH with strain RS(T) above the species cut-off values of 96 and 70%, respectively. The ANIb and dDDH values between the genome sequences from 12 strains of R. solanacearum phylotype III (Current R. pseudosolanacearum) and those of strain RS(T) were below the species cut-off values. Based on these data, we concluded that strains of phylotype I, including RS(T), represent a novel species of the genus Ralstonia, for which the name Ralstonia nicotianae sp. nov. is proposed. The type strain of Ralstonia nicotianae sp. nov. is RS(T) (=GDMCC 1.3533(T) = JCM 35814(T)).