Development of a scheme for genotyping Helicobacter pylori based on allelic variation in urease subunit genes

基于尿素酶亚基基因等位基因变异的幽门螺杆菌基因分型方案的开发

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Abstract

Helicobacter pylori urease subunit genes in 383 isolates from 10 countries were investigated by PCR-restriction fragment length polymorphism (HaeIII) analysis. Eighty-two different ureAB profiles were documented by reference to known sequences. Variation among 51% of strains was accounted for by 10 predominant patterns, which provided a unique framework for categorizing isolates with geographically diverse origins.

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