A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

一种用于快速分型主要葡萄球菌盒式染色体mec元件的新型多重实时PCR检测方法

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Abstract

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

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