Abstract
The binding of the novel cytotoxic acridine derivative, 1-[2-(acridin-9-ylamino)ethyl]-1,3-dimethylthiourea (ACRAMTU) to various self-complementary oligonucleotide duplexes has been studied by combined high-resolution NMR spectroscopy/restrained molecular dynamics and equilibrium binding assays to establish the sequence and groove specificity of intercalation. The binding mode in the sequences d(GGACGTCC)(2) and d(GGAGCTCC)(2) was deduced from chemical shift changes and intermolecular NOEs between the ligand and the oligonucleotides. ACRAMTU intercalated into the 5'-CG/CG and 5'-GA/TC base steps, and penetration of the duplexes occurred from the minor groove. Intercalation of ACRAMTU in d(GGTACC)(2) occurs at the central TA/TA step, based on the absence of the internucleotide A4H8-T3H1' and A4H8-T3H3' cross-peaks in the 1:1 complex of this sequence. An energy- minimized AMBER model of the 1:2 complex, [d(GGAGCTCC)(2)(ACRAMTU)(2)], was generated, which was based on restricted molecular dynamics/ mechanics calculations using 108 NOE distance restraints (including 11 DNA-drug distances per ligand). Equilibrium dialysis experiments were performed using octamers containing various base steps present in the 'NMR sequences'. The highest affinity for ACRAMTU was observed in d(TATAT ATA)(2), followed by d(CGCGCGCG)(2) and d(GAG ATCTC)(2). The binding levels for CG/CG and GA/TC were virtually the same. The unusual tolerance of the GA/TC intercalation site and the pronounced groove specificity of ACRAMTU play a significant role in the molecular recognition between the corresponding platinum conjugate, Pt-ACRAMTU, and DNA.