Abstract
Growing nanotechnology use in agriculture can transform traditional practices. This study investigated the application of zinc oxide nanoparticles (ZnO NPs) in Hibiscus syriacus L. cultivation, examining: particle size (30, 50, and 90 nm), concentration (10, 50, 100, 500, and 1000 mg/L), and application method (seed priming and foliar application). In seed priming experiments, low concentrations (10-100 mg/L) of all tested NP sizes enhanced germination parameters, while higher concentrations showed inhibitory effects. The optimal treatment was 30 nm ZnO NPs at 50 mg/L, achieving a 68.89% germination rate. Seed priming also significantly improved seedling growth and physiological biochemistry. In pot experiments, foliar application studies showed that while both ZnSO(4) and ZnO NPS increased leaves Zn content, nanoparticle treatments (especially smaller particles at 30 and 50 nm) produced more sustained growth benefits, with ZnO NPS outperforming traditional zinc fertilizers, particularly at 50 mg/L with 50 nm particles. These findings highlight dual advantages of ZnO NPs as an effective seed priming and foliar application, demonstrating potential as an efficient fertilizer for ornamental woody plants. In addition, significant concentration thresholds were found in both application methods (with varying thresholds), above which phytotoxic effects were observed.