Abstract
The prevalence of MDR, XDR and PDR gram-negative bacteria, drug resistance and sensitivity patterns in the intensive care unit of the tertiary care hospital Amalapuram from June 2023 to July 2024 is of interest. The study included all patients who were admitted to the intensive care unit and various clinical samples like blood, urine, respiratory samples, body fluids and pus received in the lab were inoculated on different media like Mac-Conkey agar and blood agar according to Standard Microbiological methods. Bacterial identification was done based on colony morphology, Gram stain and various biochemical reactions after obtaining pure growth. Gram-negative bacteria were identified using a series of biochemical tests, like oxidase, catalase, indole production, methyl red, V-P, citrate, urease and triple sugar iron agar tests. Antibiotic susceptibility testing was performed using the Kirby-Bauer disk diffusion method according to CLSI guidelines. We found a high frequency of antibiotic resistance in ICU isolates, primarily caused by gram-negative bacilli like Klebsiella and E. coli.