In vitro skin anti-aging potential evaluation of methanolic extract of aerial parts from Acalypha indica Linn

体外评价印度铁苋菜(Acalypha indica Linn)地上部分甲醇提取物的抗皮肤衰老潜力

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Abstract

The methanolic extract of aerial parts from Acalypha indica (MAIE) as potential candidates for skin aging treatment is of interest. The skin antiaging property was evaluated by its antioxidant and extracellular matrix degrading enzyme-inhibiting activity. The study reported the highest total phenolic content and total flavonoid content with notable antioxidant activity (ABTS and DPPH assay) at the concentration of 1, 2 and 5 µg/ml. The inhibitory potential of MAIE against Elastase (IC50 = 21.79 ± 0.01µg/ml) and MMP-1 (36.29 ± 0.01µg/ml), the key enzymes linked to acceleration of the aging process explicit enzyme inhibition. In vitro studies with L929 cells demonstrated that MAIE was noncytotoxic up to of 5 µg/mL, significantly reduced cell viability of irradiated cell by 48.33%, indicating its potent photoprotective capacity and exhibited effective Collagen biosynthesis by Sirius Red method. MAIE can be further developed as a natural skin antiaging agent in the cosmetics, cosmeceutical and pharmaceutical industries.

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