Abstract
The DNA-protein interactions play vital roles in the central dogma of molecular biology. Proper interactions between DNA and protein would lead to the onset of various biological phenomena like transcription, translation, and replication. However, the mechanisms of these well-known processes vary between prokaryotic and eukaryotic organisms. The exact molecular mechanisms of these processes are unknown. Therefore, it is of interest to report the comparative estimate of the different properties of the DNA binding proteins from prokaryotic and eukaryotic organisms. We analyzed the different sequence-based features such as the frequency of amino acids and amino acid groups in the proteins of prokaryotes and eukaryotes by statistical measures. The general pattern of differences between the various DNA binding proteins for the development of a prediction system to discriminate between these proteins between prokaryotes and eukaryotes is documented.