Abstract
BACKGROUND: Exhaustion of CD8(+) tumor-infiltrating lymphocytes (TILs), characterized by the overexpression of immune checkpoints (IC), is a major impediment to anti-tumor immunity. However, the exhaustion status of CD8(+)TILs in angioimmunoblastic T cell lymphoma (AITL) remains unclear. Therefore, we aimed to elucidate the exhaustion status of CD8(+)TILs in AITL and its influence on prognosis. METHODS: The correlation between CD8(+)TILs and IC expression in AITL was analyzed using single-cell RNA sequencing (n = 2), flow cytometry (n = 20), and RNA sequencing (n = 20). Biological changes related to CD8(+)TILs exhaustion at different cytotoxic T lymphocyte (CTL) levels (mean expression levels of CD8A, CD8B, GZMA, GZMB, and PRF1) in AITL were evaluated using RNA sequencing (n = 20) and further validated using the GEO dataset (n = 51). The impact of CD8 protein expression and CTL levels on patient prognosis was analyzed using flow cytometry and RNA sequencing, respectively. RESULTS: Our findings demonstrated that the higher the infiltration of CD8(+)TILs, the higher was the proportion of exhausted CD8(+)TILs characterized by the overexpression of multiple IC. This was accompanied by extensive exhaustion-related biological changes, which suggested severe exhaustion in CD8(+)TILs and may be one of the main reasons for the poor prognosis of patients with high CD8(+)TILs and CTL. CONCLUSION: Our study comprehensively reveals the exhaustion status of CD8(+)TILs and their potential negative impact on AITL prognosis, which facilitates further mechanistic studies and is valuable for guiding immunotherapy strategies.