Metagenomic Next-Generation Sequencing Diagnosis of Streptococcus agalactiae Meningitis in a Diabetic Patient

利用宏基因组学二代测序技术诊断糖尿病患者的无乳链球菌性脑膜炎

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Abstract

Streptococcus agalactiae meningitis is primarily observed among neonates and is uncommon in adults. We present a rare case of Streptococcus agalactiae meningitis in an adult. The patient was a 74-year-old male with a history of gastric perforation surgery, chemotherapy for neck lymphoma, and hypertension. He presented to the emergency department with an 11-day history of neck pain, one day of limited mouth opening and dysphagia, and eight hours of altered consciousness. On examination, he exhibited impaired consciousness (Glasgow Coma Scale score: 8) and cervical rigidity. He was intubated and received oxygen therapy. Laboratory findings revealed elevated infection markers and turbid cerebrospinal fluid (CSF). Metagenomic next-generation sequencing (mNGS) of the CSF detected Streptococcus agalactiae. Enhanced MRI of the head and neck showed a small subdural effusion, spinal cord edema at C5-C6, thickening and enhancement of the anterior and posterior longitudinal ligaments and meninges at the skull base, suggestive of Streptococcus agalactiae infection. After 10 days of anti-infective treatment with ceftriaxone, the patient's condition improved, and he was transferred to a local hospital for continued management.

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