Abstract
The study aims to develop a plant-based food gel with a unique texture using callus cells and a mixture of xanthan (X) and konjac (K) gums. The effect of encapsulation of carrot callus cells (0.1 and 0.2 g/mL) on properties of X-K hydrogels was studied using the mechanical and rheological analysis with a one-way ANOVA and Student's t-test used for statistical analysis. Hedonic evaluation and textural features were obtained from 35 volunteers using a nine-point hedonic scale and a 100 mm visual analog scale with the Friedman's test and the Durbin post hoc test used for statistical analysis. Mechanical hardness, gumminess, and elasticity increased by 1.1-1.3 and 1.1-1.8 times as a result of encapsulation 0.1 and 0.2 g/mL cells, respectively. The addition of cells to the hydrogels resulted in an increase in the complex viscosity, strength, and number of linkages in the gel. The hydrogel samples received identical ratings for overall and consistency liking, as well as taste, aroma, and texture features. However, the callus cell-containing hydrogel had a graininess score that was 82% higher than the callus cell-free hydrogel. The obtained hydrogels based on gums and immobilized carrot callus cells with unique textures may be useful for the development of diverse food textures and the production of innovative functional foods.