Abstract
In the tenth year since the first edition of MISEV was released in 2014, MISEV2023 has been reported in 2024 with the aim of refining the standard and improving the rigor, reproducibility, and transparency of extracellular vesicle (EV) research to clarify the requirements for experimental design of EVs, emphasize the importance of reproducible experimental results as well as encouraging openness of experimental information. The release of MISEV has significantly contributed to the quality of research in the field of EVs, which creates a more reliable research environment. However, despite the important role of MISEV, there is still a need for the EV researchers to continue to push for the widespread implementation of the guidelines to meet the evolving nature and challenges of EV research. The evolution of EV research and the attention it receives have grown exponentially over time, as has the number of people involved in the writing of MISEV. Here, this review briefly summarizes the evolution of the three editions of MISEV, aiming to recall MISEV2014 and MISEV2018 while learning about the latest release, MISEV2023, to gain a deeper understanding of the content, and to provide a quick note for beginners who want to learn about MISEV and explore the EV world.