Abstract
During mitosis in higher eukaryotic cells, nuclear envelope breakdown (NEBD) occurs and leads to the disassembly of the nuclear membrane and nuclear pore complexes (NPC). This brings about a mixing of nuclear and cytoplasmic macromolecules (open mitosis). On the other hand, in many fungi, mitosis occurs without NEBD (closed mitosis). In a recent study, we reported a novel phenomenon in a closed mitosis organism, Schizosaccharomyces pombe: mixing of nuclear and cytoplasmic proteins occurred in meiosis without breakdown of the nuclear membrane or disassembly of nuclear pore complexes. We designated this event virtual nuclear envelope breakdown (V-NEBD). The key event in V-NEBD is nuclear translocation of Rna1, a RanGAP1 homologue in S. pombe. This leads to collapse of the Ran-GTP gradient across the nuclear envelope (NE) and occurs coincidently with V-NEBD. Thus, the barrier function of the NE can be abated without its physical breakdown through modulation of the Ran-GTP gradient.