Neuromuscular electrical stimulation resistance training enhances oxygen uptake and ventilatory efficiency independent of mitochondrial complexes after spinal cord injury: a randomized clinical trial

神经肌肉电刺激阻力训练可提高脊髓损伤后患者的氧气摄取量和通气效率,且该作用独立于线粒体复合物:一项随机临床试验

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Abstract

The purpose of the study was to determine whether neuromuscular electrical stimulation resistance training (NMES-RT)-evoked muscle hypertrophy is accompanied by increased V̇o(2) peak, ventilatory efficiency, and mitochondrial respiration in individuals with chronic spinal cord injury (SCI). Thirty-three men and women with chronic, predominantly traumatic SCI were randomized to either NMES-RT (n = 20) or passive movement training (PMT; n = 13). Functional electrical stimulation-lower extremity cycling (FES-LEC) was used to test the leg V̇o(2) peak, V̇E/V̇co(2) ratio, and substrate utilization pre- and postintervention. Magnetic resonance imaging was used to measure muscle cross-sectional area (CSA). Finally, muscle biopsy was performed to measure mitochondrial complexes and respiration. The NMES-RT group showed a significant increase in postintervention V̇o(2) peak compared with baseline (ΔV̇o(2) = 14%, P < 0.01) with no changes in the PMT group (ΔV̇o(2) = 1.6%, P = 0.47). Similarly, thigh (ΔCSA(thigh) = 19%) and knee extensor (ΔCSA(knee) = 30.4%, P < 0.01) CSAs increased following NMES-RT but not after PMT. The changes in thigh and knee extensor muscle CSAs were positively related with the change in V̇o(2) peak. Neither NMES-RT nor PMT changed mitochondrial complex tissue levels; however, changes in peak V̇o(2) were related to complex I. In conclusion, in persons with SCI, NMES-RT-induced skeletal muscle hypertrophy was accompanied by increased peak V̇o(2) consumption which may partially be explained by enhanced activity of mitochondrial complex I.NEW & NOTEWORTHY Leg oxygen uptake (V̇o(2)) and ventilatory efficiency (V̇E/V̇co(2) ratio) were measured during functional electrical stimulation cycling testing following 12-16 wk of either electrically evoked resistance training or passive movement training, and the respiration of mitochondrial complexes. Resistance training increased thigh muscle area and leg V̇o(2) peak but decreased V̇E/V̇co(2) ratio without changes in mitochondrial complex levels. Leg V̇o(2) peak was associated with muscle hypertrophy and mitochondrial respiration of complex I following training.

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