Abstract
Silver nanoparticles (AgNPs) are widely used as antibacterial agents either as colloidal solutions or deposited on surfaces. However, the high concentration of AgNPs can lead to cytotoxicity, posing a hazard to healthy cells and tissues. Achieving a balance between antibacterial efficacy and cytocompatibility is crucial for biomedical applications. Polymeric coatings, especially those made from polydimethylsiloxane (PDMS) like Sylgard 184, are popular in biomedical applications due to their user-friendliness. We have developed a cost-effective method to reduce silver ions using the Si-H silane functions of PDMS in situ. Tetrahydrofuran (THF) acts as a solvent, inducing a swelling effect in PDMS, allowing silver ions from silver tetrafluoroborate (AgBF(4)) dissolved in THF to diffuse into the polymer and undergo reduction. This process results in PDMS functionalized with well-distributed 10 nm silver AgNPs. The resulting metal-polymer nanocomposites (MPNs) exhibit yellow shades and, based on qualitative Live/Dead staining observations, show no apparent cytotoxicity on human gingival fibroblasts. In addition, SEM analyses indicate a qualitative reduction in E. coli adhesion, suggesting an antibacterial anti-adhesive potential against this bacterial strain. Further studies should investigate the release profile of AgNPs in these composites, which could guide the development of new biocompatible coatings for phototherapy devices and enhance their long-term clinical performance.