Development of RNA-Based Assay for Rapid Detection of SARS-CoV-2 in Clinical Samples

开发基于 RNA 的临床样本中 SARS-CoV-2 快速检测方法

阅读：6

作者：Vinod Kumar, Suman Mishra, Rajni Sharma, Jyotsna Agarwal, Ujjala Ghoshal, Tripti Khanna, Lokendra K Sharma, Santosh Kumar Verma, Prabhakar Mishra, Swasti Tiwari

期刊：

Intervirology

影响因子：

3.200

时间：

2022

起止号：

2022;65(4):181-187.

doi：

10.1159/000522337

研究方向：

代谢、免疫、心血管

疾病类型：

新冠

Conclusion

We found that the developed assay rapidly detects SARS-CoV-2 RNA in clinical samples in a cost-effective manner and would be useful in pandemic management by facilitating mass screening.

Results

The results were validated using nasopharyngeal RNA samples from positive COVID-19 subjects (n = 136). Using real-time polymerase chain reaction as gold standard, the assay was found to have 85.29% sensitivity and 94.12% specificity. The optimized method has detection limit as little as 0.5 ng of SARS-CoV-2 RNA.

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