Abstract
Graphdiyne (GDY), the sole synthetic carbon allotrope with sp-hybridized carbon atoms, has been extensively researched that benefit from its pore structure, fully conjugated surfaces, wide band gaps, and more reactive C≡C bonds. In addition to the intrinsic features of GDY, engineering at the nanoscale, including metal/transition metal ion modification, chemical elemental doping, and other biomolecular modifications, endowed GDY with a broader functionality. This has led to its involvement in biomedical applications, including enzyme catalysis, molecular assays, targeted drug delivery, antitumor, and sensors. These promising research developments have been made possible by the rational design and critical characterization of GDY biomaterials. In contrast to other research areas, GDY biomaterials research has led to the development of characterization techniques and methods with specific patterns and some innovations based on the integration of materials science and biology, which are crucial for the biomedical applications of GDY. The objective of this review is to provide a comprehensive overview of the biomedical applications of GDY and the characterization techniques and methods that are essential in this process. Additionally, a general strategy for the biomedical research of GDY will be proposed, which will be of limited help to researchers in the field of GDY or nanomedicine.