Abstract
Nano-hydroxyapatite/polyamide 66 (nHA/PA66) composite with good bioactivity and osteoconductivity was employed to develop a novel porous membrane with asymmetric structure for guided bone regeneration (GBR). In order to test material cytotoxicity and to investigate surface-dependent responses of bone-forming cells, the morphology, proliferation, and cell cycle of bone marrow stromal cells (BMSCs) of rats cultured on the prepared membrane were determined. The polygonal and fusiform shape of BMSCs was observed by scanning electronic microscopy (SEM). The proliferation of BMSCs cultured on nHA/PA66 membrane tested by the MTT method (MTT: [3-{4,5-dimethylthiazol-2yl}-2,5-diphenyl-2H-tetrazoliumbromide]) was higher than that of negative control groups for 1 and 4 days' incubation and had no significant difference for 7 and 11 days' culture. The results of cell cycle also suggested that the membrane has no negative influence on cell division. The nHA/PA66 membranes were then implanted into subcutaneous sites of nine Sprague Dawley rats. The wounds and implant sites were free from suppuration and necrosis in all periods. All nHA/PA66 membranes were surrounded by a fibrous capsule with decreasing thickness 1 to 8 weeks postoperatively. In conclusion, the results of the in vitro and in vivo studies reveal that nHA/PA66 membrane has excellent biocompatibility and indicate its use in guided tissue regeneration (GTR) or GBR.