Re description and molecular identification of Thalamita danae (Stimpson 1858) (Decapoda: Brachyura: Portunidae) based on fresh material from the coastal waters, Pakistan

基于巴基斯坦沿海水域新鲜材料对 Thalamita danae (Stimpson 1858)(十足目:短尾亚纲:梭子蟹科)进行重新描述和分子鉴定

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作者:Noor Us Saher, Farah Naz, Syeda Mariam Siddiqa, Mohammad Mumtaz Khan, Mustafa Kamal

Abstract

Thalamita danae (Stimpson1858), re-described as a new report, based on an integrative taxonomy approach combining 16S rRNA partial sequence of mitochondrial DNA and morphological analyses was used for the accurate identification of specimens. The morphological and molecular analysis provides the confirm evidence of T. danae in coastal waters of Pakistan. Results were confirmed by amplification of partial sequences of 16S rRNA mt-DNA gene and the sequence was searched for similarity using BLASTn (Basic Local Alignment Tool), the result showed 97% sequence similarity with partial sequences of T. danae Stimpson, 1858 as obtained from gene bank. The attained sequence was submitted to gene bank after confirmation of genetic and morphological similarity. High sequence similarity with accession no: FJ152165.1 indicated, that misidentification of species does not occur.

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