Abstract
In 1997 with the S. cerevisiae genome about to be completed, a multidisciplinary research resource center was proposed to NIH. This center would combine multiple technologies to investigate the biology of yeast in collaboration with yeast biologists. One technology to be used in the center was mass spectrometry as the use of sequence databases allowed the accurate and rapid identification of proteins. Initial use of mass spectrometry was to identify proteins from gels which quickly evolved to a shotgun approach for the identification of proteins in mixtures. Collaborations have been combined with technology development to advance the ability to address new questions. The evolution of technology with some collaboration highlights will be discussed during the talk.