Abstract
Cleavage Under Targets & Release Using Nucleases (CUT&RUN) sequencing is a technique used to study gene regulation. The protocol presented here has been used successfully to identify the pattern of histone modifications within the genome of the eye-antennal disc of the fruit fly, Drosophila melanogaster. In its present form, it can be used to analyze genomic features of other imaginal discs. It can be modified for use with other tissues and applications including identifying the pattern of transcription factor occupancy.
Keywords:
Bioinformatics; Developmental biology; Genetics; Genomics; Model Organisms; Molecular Biology; Protein Biochemistry; Protein expression and purification; Proteomics; Sequence analysis.