Abstract
Background. Microbial accumulation is still a significant problem with removable acrylic appliances. This study aimed to assess the antimicrobial properties of a self-cured acrylic resin containing curcumin nanoparticles (CNPs). Methods. This in vitro study used 48 acrylic discs containing 0.5%, 1%, and 2% CNPs. The antimicrobial properties of the discs against Streptococcus mutans, Streptococcus sanguinis, Lactobacillus acidophilus, and Candida albicans were evaluated using disc agar diffusion (DAD), eluted component, and biofilm inhibition tests. The growth inhibition zones were measured, and the colonies were counted after 1, 3, and 7 days. Results. DAD test showed that none of the curcumin nanoparticle concentrations caused growth inhibition zones for any microorganisms. All the concentrations were effective against all four microorganisms in the biofilm inhibition test except 0.5% for L. acidophilus. In the eluted component test, solutions containing 2% concentration had maximum growth inhibition of all the groups at all time intervals. An increase in curcumin nanoparticle concentration from 0.5% to 1% was effective only against C. albicans. Conclusion. Generally, CNPs in all concentrations were effective against the biofilms of all four microorganisms assessed in this study. Therefore, incorporating 2% CNPs into acrylic resin seems suitable for clinical use.