Observations on the utilization of a selective medium for the isolation of Erysipelothrix rhusiopathiae

关于利用选择性培养基分离猪丹毒杆菌的观察

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Abstract

A medium containing 3 antibiotics: neomycin, kanamycin and vancomycin for the selective culture of Erysipelothrix rhusiopathiae was studied during various culture tests. It was found that 6 out of 35 previously identified strains of E. rhusiopathiae (17 %) showed no growth or variable growth on the medium. It appeared that the strains with the highest sensitivity for kanamycin were inhibited by the medium. A number of other laboratory strains of microorganisms did not grow on the medium, indicating a good selective effect. Several resistant microorganisms were, however, found in field material. A small number of isolation attempts using tonsils of swine and sheep revealed a very low occurrence (4 % in swine) of E. rhusiopathiae.

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