Immunocytochemical localization of tubulin, actin, and myosin in axonemes of ciliated cells from quail oviduct

利用免疫细胞化学方法对鹌鹑输卵管纤毛细胞轴丝中的微管蛋白、肌动蛋白和肌球蛋白进行定位。

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Abstract

Tubulin, actin, and myosin have been localized in isolated demembranated ciliated cells from quail oviduct by immunocytochemistry in both light and electron microscopy by using purified antibodies. The peripheral doublets and the central tubules are stained by the antitubulin whereas the kinetosomes are poorly stained. Actin antibodies clearly stain the axonemes, but only on the proximal-half portion, whereas myosin antibodies stain a small area of the axonemes just above the ciliary neck region.

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