Abstract
Coated vesicles prepared by using isotonic gradients retain their contents. Ferritin, transferrin, and immunoglobulin G have been detected in human placental coated vesicles. Triton X-100 extraction of crude coated vesicles removes contaminating uncoated membranes, leaving coated particles and their associated proteins apparently intact; this provides an efficient means of preparing large quantities of coated vesicle proteins. The clathrin cages surround "cores" of structural protein containing polypeptides of 100,000 and 50,000 molecular weight.