The effects of curing agents on the proteolysis and lipid oxidation of pastırma produced by the traditional method

腌制剂对传统方法制作的帕斯特玛(pastırma)中蛋白质水解和脂质氧化的影响

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Abstract

In the study, the effects of nitrate and/or nitrite (150 mg/kg KNO(3), 300 mg/kg KNO(3), 150 mg/kg NaNO(2), and 150 mg/kg KNO(3) + 150 mg/kg NaNO(2)) on proteolytic changes (free amino acid composition, SDS-PAGE) in pastırma were investigated. Pastırma samples were also analyzed in terms of some qualitative (pH, a(w), TBARS, residual nitrite, salt) properties. The lowest total free amino acid content (1818.3 mg/ 100 g DM) was observed in the combination of 150 mg/kg KNO(3) + 150 mg/kg NaNO(2), while the highest content (2847.49 mg/ 100 g DM) was observed in 150 mg/kg KNO(3). Although the pastırma groups generally exhibited similar SDS-PAGE profiles, differences were detected at some band intensities. The lowest TBARS value (22.24 μmol MDA/kg) was observed in 150 mg/kg KNO(3) + 150 mg/kg NaNO(2). As a result, the use of 150 mg/kg KNO(3) in the pastırma curing process causes more intense proteolysis.

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