Abstract
Chitin is the second-largest natural polymer polysaccharide in nature. Due to its important physical and chemical properties and excellent biocompatibility, safety, and biodegradability, it is widely used in agriculture, medicine, food, environmental protection, and other fields. However, traditional extraction methods cause environmental pollution and damage the structure of chitin. Bioprocessing is an emerging technology that shows great potential. In this research, the puparia and adults of black soldier fly (BSF) (Hermetia illucens L.) were used as raw materials. A continuous fermentation method was designed to extract chitin, by using Bacillus subtilis S4 and Acetobacter pasteurianus AS1.41. The Fourier transform infrared spectroscopy identification results showed that the extracted sample was α-chitin. Under continuous fermentation conditions, the deproteinization (DP) rate, demineralization (DM) rate, chitin yield (CY), and deacetylation degree (DD) of puparium chitin were 33.33%, 94.92%, 59.90%, and 18.52%, respectively. Meanwhile, the DP rate, DM rate, CY, and DD of adult chitin were 46.63%, 90.93%, 47.31%, and 37.38%, respectively. For BSF, B. subtilis S4 had a certain DP ability, and A. pasteurianus AS1.41 had a good DM effect. Moreover, BSF at different developmental stages could affect CY, and a higher concentration of NaOH was more favorable for deacetylation. Overall, simultaneous continuous fermentation could be a new biological approach to extract chitin from BSF.