Abstract
Selenium has strong antioxidant properties and diverse effects on the immune system. The aim of the study was to analyse the protective effect of selenium as a component of a kidney preservation solution on the prevention of ischemia-reperfusion injury of nephrons. The solution was modified by the addition of Se (1 µg/L), prolactin (0.1 µg/L) and Se with prolactin (1 µg/L Se + 0.1 µg/L PRL). The study used a model for storing isolated porcine kidneys in Biolasol(®) (modified Biolasol(®)), which minimizes ischemia-reperfusion injury of grafts. The introduction of Se(4+) ions at a dose of 1 µg/L into the Biolasol(®) preservation solution in the form of Na(2)SeO(3) caused an increase in the activity/concentration of the analysed biochemical parameters: aspartate transaminase, alanine transaminase, urea and protein. This suggests an adverse effect of Se(4+) on nephron function during ischemia-reperfusion. The best graft protection was obtained by using Biolasol(®) modified with the addition of selenium (IV) at a dose of 1 µg/L and prolactin at a concentration of 0.1 µg/L. We proposed the mechanism of prolactin action in the metabolic reduction of selenite (SO(3)(2-)) during ischemia/reperfusion.