Abstract
Alkane is a predominant wax component, whose production requires the aids of CER1 and CER3. In rice, OsCER1 and OsCER3 are present in multiple copies. Until now, the roles of these genes have been studied individually; however, a systematic comparison of their relative contributions to cuticular wax biosynthesis has not yet been carried out. Phylogenetic tree analysis revealed that CER1s and CER3s from different plants are classified into two subgroups. RT-qPCR analysis showed that these genes display distinct expression patterns, revealing their specific roles in wax production. Promoter prediction analysis showed that cis-elements responding to light, phytohormones and stress are enriched in the promoter region of OsCER1s and OsCER3s. These proteins are all localized in the endoplasmic reticulum. Further study showed that OsCER1s and OsCER3s are inclined to form a complex during the wax synthesis. Finally, the wax analysis of single mutants showed that among the examined genes, OsCER3a mutation greatly reduced the total wax amounts to 19.6% of wild-type plant with a decrease in most of wax components, whereas mutation of other genes including OsCER3b, OsCER3c, OsCER1a and OsCER1c slightly or barely affect wax production, suggesting that OsCER3a plays major roles in rice wax production whereas other proteins redundantly participate in the wax synthesis. Additionally, the wax increasing rates of Arabidopsis expressing OSCER1 are lower than those of overexpressing AtCER1. Taken together, our study identified the predominant genes involved in wax production, which will be useful for genetically engineering rice with enhanced stress tolerance.