Abstract
In order to examine the seasonal variations in the morphological characteristics and diet of Mactra veneriformis in the Northern Yellow River Delta's intertidal zone and provide a scientific basis for its resource conservation and population restoration, tested clams were collected in four consecutive seasons from summer of 2022 to spring of 2023. Morphological traits were measured, and the DNA of the stomach contents was analyzed using high-throughput sequencing. The tidal differences and seasonal variations in the northern habitat of the Yellow River Estuary significantly affect the morphological characteristics and growth of M. veneriformis. Among the four seasons, significant differences in the morphological characteristics of M. veneriformis were observed between the middle-tide and low-tide zones (p < 0.05). In both middle-tide and low-tide zones, the morphological characteristics and body wet weight of M. veneriformis in winter were significantly higher than those in other seasons (p < 0.05). Moreover, the morphological characteristics of M. veneriformis were extremely significantly influenced by the interaction between tide and season (two-way interaction, p < 0.001). In all seasons, M. veneriformis in the middle- and low-tide zones exhibited positive allometric growth. While there was no significant difference in the stomach content between the spring and summer samples in the same tidal zone (p > 0.05), there was a significant difference between the middle- and low-tide samples in winter (p < 0.05). This suggests that seasonal variations, rather than tidal differences, had a larger impact on the diet of M. veneriformis in the northern Yellow River estuary and that feeding differences may be related to changes in environmental factors, such as temperature. The findings of this study provide initial insights into the feeding ecology of M. veneriformis and offer a scientific foundation for the conservation and management of its resources.