A protocol for simultaneous in vivo juxtacellular electrophysiology and local pharmacological manipulation in mouse cortex

Here, we describe a protocol to simultaneously record and label single cortical neurons in vivo under local application of a chemical such as a receptor agonist. This protocol provides a useful tool to investigate how the chemical of interest affects the processing of sensory information by cortical neurons. The juxtacellular labeling allows identification of the cell type and morphology of the recorded neurons. We draw examples to show pharmacological modulations in encoding of vibrotactile stimuli in the mouse primary somatosensory cortex. For complete details on the use and execution of this protocol, please refer to Kheradpezhouh et al. (2020).