Cell type-specific response to curvature controls tissue growth dynamics in biomaterial pores

细胞类型特异性的曲率反应控制着生物材料孔隙中的组织生长动力学

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Abstract

In porous biomaterials, pore diameter and surface curvature are critical parameters governing cell migration, multicellular organization, and subsequent tissue formation, ultimately influencing in vivo performance. However, how different cell types respond to curvature and what determines their specific adaptations remain poorly understood. Here, we expose diverse cell types to precisely controlled, physiologically relevant microgeometries, revealing two distinct cellular strategies for adapting to curvature on channel-like surfaces. We examine how cellular stress modulation and the induction of senescence influence curvature responses and identify a universal predictor of curvature adaptation, linked to focal adhesion distribution across cell types and stress states. Consequently, tissue growth dynamics and final tissue architecture vary significantly between cell types. This mechanistic insight enables the prediction of optimal biomaterial pore diameters for specific cell types, offering a valuable framework for designing biomaterials with tailored cell-instructive properties for targeted tissue engineering applications.

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