Abstract
Dendritic cells (DCs) are antigen-presenting cells that play an important role in connecting the innate and adaptive immunity of the immune system. To mediate innate and adaptive immunity, DCs pass through two stages: immature and mature. The change of phenotype is closely associated with the morphological and functional characteristics of DCs. Understanding these properties of DCs is important in the context of recent efforts on the developments of biomaterials-based cancer vaccine. In this paper, the morphological and phenotypical status of DCs in both stages were compared, and their relationship to the phagocytic and migratory ability of the cells was studied using bone-marrow derived dendritic cells (BMDCs). Immature DCs were of a circular shape and expressed low levels of costimulatory molecules, while mature DCs had longer dendrites and expressed high levels of costimulatory molecules. The phagocytic and migratory ability studied using the polymer bead uptake test and live imaging indicated that immature DCs have a pronounced phagocytic ability compared to mature DCs, while the mature DCs moves faster than immature DCs. These findings could be helpful for understanding the relationship between immature and mature DCs and analyzing initiation of the adaptive immune response by DCs in DC-mediated immunotherapy.