Abstract
Raman and Brillouin spectroscopy enable non-invasive assessment of chemical and elastic properties of biomaterials, respectively. In this report, Brillouin micro-spectroscopy was used for the time-resolved analysis of elastic properties of Populus and Geranium leaves, while Raman micro-spectroscopy was employed for the assessment of their chemical variation during drying. Spectroscopic assessment of elastic and chemical properties can improve our understanding of mechano-chemical changes of plants in response to environmental stress and pathogens at the microscopic cellular level. This report demonstrates the potential of multimodal optical sensing and imaging of plants as an emerging technique for the quantitative assessment of agricultural crops.