Abstract
Despite its numerous advantages, the aerobic granular sludge (AGS) process faces several challenges that hinder its widespread implementation. One such challenge is the requirement for high organic load ratios (OLR), which significantly impacts AGS formation and stability, posing a barrier to commercialization. In response to these challenges, this study investigates the granulation and treatment efficacy of the AGS process for treating high-concentration wastewater under various OLR and settling time. Three sequential batch reactors (R1, R2, R3) were operated at OLRs of 0.167, 0.33, and 1 kg COD/m(3)·day. The study focuses on analyzing key parameters including sludge characteristics, extracellular polymeric substances (EPS) content, PN/PS ratio, and microbial clusters. Results demonstrate that reducing settling time from 90 to 30 min enhances sludge settleability, resulting in a maximum 50.8 % decrease in SVI(30) (from 98.1 to 122.8 mL/g to 51.9-81.3 mL/g), thereby facilitating the selection of beneficial microorganisms during granulation. Particularly, at R2, the PN/PS ratio was 4.3, and EPS content increased by 1.52-fold, leading to a 1.41-fold increase in sludge attachment. This observation suggests a progressive maturation of AGS. Additionally, analysis of microbial diversity and cluster composition highlights the influence of OLR variations on the ratios of Proteobacteria and Bacteroidetes. These findings emphasize the significant impact of SBR operational strategies on AGS process performance and biological stability, offering valuable insights for the efficient operation of future high-concentration wastewater treatment processes.