Neurotoxicological examination of the piglet brain after prenatal and postnatal exposure to trichlorfon

对产前和产后暴露于敌百虫的仔猪脑进行神经毒理学检查

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Abstract

Piglets were given 10 repeated doses of the orga-nophosphorus compound trichlorfon during the postnatal period in order to examine the effect on the brain development (Experiment 1). Following prenatal exposure to trichlorfon, the ability of a presumptive hypoplastic cerebellum and cerebrum at birth, to regenerate postnatally was investigated (Experiment 2). Administration of repeated doses of trichlorfon postnatally was accompanied by only small changes in brain weights, morphology and transmitter enzyme activity (choline acetyltransferase, glutamate decarboxylase, aromatic amino acid decarboxylase) in 35 days old piglets. Animals exposed prenatally, and sacrificed at the age of 35 days, showed a significant increase in brain weights and enzyme activities. The animals did, however, not reach control values in cerebral weight, cerebellar weight or total enzyme activities. Morphological changes still showed regional loss of Purkinje cells in the cerebellum. The study clearly indicated that the pig brain was less vulnerable to trichlorfon in the postnatal period of development than when exposed to the compound prenatally.

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