Abstract
BACKGROUND: Fluorescence microscopy (FM) is an important and widely adopted biological imaging technique. Segmentation is often the first step in quantitative analysis of FM images. Deep neural networks (DNNs) have become the state-of-the-art tools for image segmentation. However, their performance on natural images may collapse under certain image corruptions or adversarial attacks. This poses real risks to their deployment in real-world applications. Although the robustness of DNN models in segmenting natural images has been studied extensively, their robustness in segmenting FM images remains poorly understood RESULTS: To address this deficiency, we have developed an assay that benchmarks robustness of DNN segmentation models using datasets of realistic synthetic 2D FM images with precisely controlled corruptions or adversarial attacks. Using this assay, we have benchmarked robustness of ten representative models such as DeepLab and Vision Transformer. We find that models with good robustness on natural images may perform poorly on FM images. We also find new robustness properties of DNN models and new connections between their corruption robustness and adversarial robustness. To further assess the robustness of the selected models, we have also benchmarked them on real microscopy images of different modalities without using simulated degradation. The results are consistent with those obtained on the realistic synthetic images, confirming the fidelity and reliability of our image synthesis method as well as the effectiveness of our assay. CONCLUSIONS: Based on comprehensive benchmarking experiments, we have found distinct robustness properties of deep neural networks in semantic segmentation of FM images. Based on the findings, we have made specific recommendations on selection and design of robust models for FM image segmentation.