Abstract
Illumination of biological events with near-infrared II sub-channel (NIR-IIb, 1500-1700 nm) enhances the transparency of biological tissues, which is very attractive for deep imaging. Due to the long-wavelength, which reduces optical damage, suppresses autofluorescence, and obviates light scattering, NIR-IIb nanoprobes afford deep tissue penetration with unprecedented spatiotemporal resolution. Hence, NIR-IIb imaging facilitates deep learning and decipherment of biological proceedings in living organisms with astounding high clarity. In comparison to its predecessors in the visible-near-infrared spectrum, imaging in the NIR-IIb has shown great potential for tissue imaging and extrapolating imaging applications for clinical studies. However, the use of organic fluorescent nanoprobes (OFNPs) in the NIR-IIb region is still rare since it is in its early stages. Thus, herein we aim to survey the recent development of different organic fluorescent nanomaterials with NIR-IIb characteristics, their unique photophysical properties, and their utilization in deep imaging in animal models. Further, practical researches on organic fluorescent nanoprobes with NIR-IIb emission and their transition to clinical applications are highlighted.