The Evaluation of Anti-Osteoclastic Activity of the Novel Calcium Hydroxide Biodegradable Nanoparticles as an Intracanal Medicament

新型氢氧化钙可生物降解纳米颗粒作为根管内药物的抗破骨细胞活性评价

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Abstract

OBJECTIVE: The present investigation looked into PLGA micron-sized particles or NPs, loaded with calcium hydroxide for canal disinfecting processes which have antimicrobial properties, given the significant rejection probabilities related to these kinds of treatments. METHODOLOGY: The MIC and inhibitory growing zones (n = 5) were found using the broth dilution and agar diffusion tests, accordingly. Employing confocal microscopy and live/dead labelling, cell viability was evaluated (n = 5). ANOVA was used to evaluate, and post hoc analysis came next. RESULTS: Ca(OH)(2) NPs showed a minimum inhibitory concentration of 5 μg/mL for Fn and 10 μg/mL for Pg and Ef (P < 0.001), following a 24-hour incubation period. CONCLUSION: To sum up, Ca(OH)(2)-contained PLGA NPs show encouraging ability to kill microbes during canal cleaning treatments.z.

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