On-chip Microscopy Using Random Phase Mask Scheme

利用随机相位掩模方案的片上显微镜

阅读:3

Abstract

In this study, a simple and novel phase-retrieval scheme is implemented using multi-angle illumination to enhance the resolution of lensless microscopy. A random-phase mask (from 0 to 2π) precedes the sample to encode the information at the sensor plane. The sample is illuminated with multiple angles that are symmetrical along the optical axis of the system. The system is initially calibrated while recording the images without any sample at the corresponding multi angles. The two types of image are mutually subtracted, and the resultant images are summed at the sensor plane and backpropagated to the sample plane. The final image is free of the twin-image effect, and has a high signal-to-noise ratio owing to the multi angles of the illumination scheme. This scheme gives a resolution of ~4 micron for a large field-of-view (~15 mm(2)). The scheme is useful for robust imaging owing to the fast phase-retrieval method, and it enables a straightforward analytical reconstruction instead of using complicated iterative algorithms in a lensless microscopic setup.

特别声明

1、本页面内容包含部分的内容是基于公开信息的合理引用;引用内容仅为补充信息,不代表本站立场。

2、若认为本页面引用内容涉及侵权,请及时与本站联系,我们将第一时间处理。

3、其他媒体/个人如需使用本页面原创内容,需注明“来源:[生知库]”并获得授权;使用引用内容的,需自行联系原作者获得许可。

4、投稿及合作请联系:info@biocloudy.com。