Abstract
Hundred-micron-sized microdroplets are widely used in microbial culture, chemical investigations and industrial processes. The size, velocity and frequency of microdroplets significantly affect the cultivation and processing effects. The detections of droplets mainly rely on capacitance detection or imaging, but it requires expensive and complex systems for capacitance detection, and high-throughput imaging detections are challenging. In this study, four-way microfluid structure (FWMS) is proposed for microdroplets generation and detection. FWMS, fixed on a 3D-printed holder, is designed to generate microdroplets (100-500 µm), with optical fibers embedded to collect double photoresist method pulses of scattering light by fast-moving microdroplets. The size and volume of the microdroplets are retrieved by tracking the double pulse signal in the time sequence. In the experiments, 50 groups of microdroplets (a total of 10(5) microdroplets) with size ranging from 100 to 450 µm were generated and detected. Compared with traditional imaging detection, this method has a better sampling rate and detection error of less than 1.42%, which can provide a simple and accurate integrated microfluid system for microdroplet generation and synchronous detection.